چکیده (انگلیسی):

Within the research and development environment, higher throughput, parallelized protein purification is
required for numerous activities, from small scale purification of monoclonal antibodies (mAbs) and antibody
fragments for in vitro and in vivo assays to process development and optimization for manufacturing. Here, we
describe specific applications and associated workflows of the Protein Maker liquid handling system utilized in
both of these contexts. To meet the requirements for various in vitro assays, for the identification and validation
of new therapeutic targets, small quantities of large numbers of purified antibodies or antibody fragments are
often required. Reducing host cell proteins (HCP) levels following capture with Protein A by evaluating various
wash buffers is an example of how parallelized protein purification can be leveraged to improve a process
development outcome. Stability testing under various conditions of in-process intermediates, as an example,
the mAb product from a clarified harvest, requires parallelized protein purification to generate concurrent samples
for downstream assays. We have found that the ProteinMaker can be successfully utilized for small-to-mid
scale platform purification or for process development applications to generate the necessary purified protein
samples. The ability to purify and buffer exchange up to 24 samples in parallel offers a significant reduction in
time and cost per sample compared to serial purification using a traditional FPLC system. By combining the
Protein Maker purification system with a TECAN Freedom EVO liquid handler for automated buffer exchange
we have created a new, integrated platform for a variety of protein purification and process development
applications.