چکیده (انگلیسی):

An efficient in vitro production of biomass hairy root cultures and elicitation of b-carboline
alkaloids of Peganum harmala was established from P. harmala seedlings. Hairy root culture
was arised by inoculation of the excised seedling with Agrobacterium rhizogenes. Analysis of rolC
and virC genes by using polymerase chain reaction (PCR) has confirmed the transformation of
the root culture. Hairy root cultures were employed to study the production of harmine as the main
b-carboline alkaloids. The transformed cultures were incubated with potential elicitors, such as
hydrogen peroxides (H2O2), or biosynthetic precursor as tryptophane in order to stimulate the biosynthesis
of b-carboline alkaloids, mainly harmine as the major one. Profile and amounts of b-carboline
alkaloids were analyzed using capillary GLC–MS.
The results revealed that, treatment of the cultures with H2O2 (0.01%) induced the accumulation of
harmine, as the major b-carboline alkaloids in root, by approximately sixfold as compared to the
untreated control. The highest stimulation resulted in a harmine level of ca. 4.5 mg/g DW after
24 h of incubation. Concerning the biosynthetic precursor, tryptophane (2 mM) enhanced the alkaloid
production approximately fivefold (0.25–0.3 mg/g DW) within 24 h.