چکیده (انگلیسی):

Different methods to create and manipulate pore sizes in hydrogel fabrication are available, but
systematic studies are normally conducted with hydrogels made of synthetic chemical compounds as
backbones. In this study, a hydrogel made of natural and abundant protein in combination with different,
well-available techniques was used to produce different architectures within the hydrogel matrix. Pore
sizes and distribution are compared and resulting hydrogel properties like swelling ratio, resistance
towards external stimuli and enzymatic degradation were investigated. Porous hydrogels were
functionalized and two cancer cell lines were successfully adhered onto the material. With simple
methods, pores with a radius between 10 and 80 mm and channels of 25 mm radius with a length of
several hundreds of mm could be created and analyzed with laser scanning confocal microscopy and
electron microscopy respectively. Furthermore, the influence of different methods on swelling ratio,
enzymatic degradation and pH and temperature resistance was observed.