چکیده (انگلیسی):

Objective: To study the effect of hypoxia on the expression of placental trophoblast cells
SATB1 and b-catenin and its correlation with the pathogenesis of preeclampsia.
Methods: Trophoblastic cell lines HRT8/SVneo were cultured, SATB1 and b-catenin
expression and cell biological behavior were determined after hypoxia reoxygenation
treatment; cell biological behavior and the expression of related genes were determined after
the transfection of SATB1 and b-catenin siRNA; preeclampsia placenta and normal placenta
tissues were collected and the expression of SATB1 and b-catenin were determined.
Results: OD value, cell migration rate, mRNA contents of SATB1 and b-catenin of H/R
group were significantly lower than those of Nor group, cell apoptosis rate was higher
than that of Nor group and the number of invasive cells was less than that of Nor group;
OD value and bcl-2 mRNA content of SATB1-siRNA group were lower than those of NC
group; cell apoptosis rate as well as Bax, Caspase-3, Caspase-6 and Caspase-9 mRNA
contents were higher than those of NC group; cell migration rate as well as CTSB, CTSD,
MMP2 and MMP9 mRNA contents of b-catenin-siRNA group were lower than those of
NC group; the number of invasive cells was less than that of NC group; the expression
levels of SATB1 and b-catenin in preeclampsia placenta tissue were significantly lower
than those in normal placenta tissue.
Conclusions: Hypoxia can inhibit the expression of SATB1 and b-catenin in the pathogenesis
of preeclampsia, which can affect the proliferation, apoptosis, migration and
invasion of cells.