چکیده (انگلیسی):

Objective: To investigate the effects of co-transfection of miR-520c-3p and miR-132 on
proliferation and apoptosis of hepatocellular carcinoma Huh7.
Methods: Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was
used to transfect miR-520c-3p and miR-132, respectively or together. The effects of
transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were
detected by CCK8 and Annexin V staining and flow cytometry, and the expression level
of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by
Western blot and realtime PCR.
Results: Compared with the control group, the proliferation ability of Huh7 of the single
transfected and co-transfected miR-520c-3p and miR-132 decreased significantly, and the
apoptosis ratio increased distinctly (P < 0.05). Besides, the effect of the co-transfection
group was better than that of the single transfection group. The protein levels of GPC3
(Glypican-3) and YAP (Yes-associated protein), the target genes transfected only by miR-
520c-3p and miR-132, respectively, reduced obviously (P < 0.05), which was similar
with the co-infected cells, but cells transfected by miR-132 only showed a decrease of
YAP.
Conclusions: The co-transfection of miR-520c-3p and miR-132 can target-regulate the
expression of GPC3 and YAP, enhance the exhibition effect on proliferation of hepatocellular
carcinoma Huh7 and induce cell apoptosis synergistically.