چکیده (انگلیسی):

Background: Toll-like receptor 3 (TLR3) is expressed in upper airways, however, little is known regarding
whether Toll-like receptor 3 (TLR3) signals exert a regulatory effect on the pathogenesis of chronic
rhinosinusitis with nasal polyps (CRSwNP), especially on eosinophilic inflammation. We sought to
investigate the effect of Poly(IC), the ligand for TLR3, on cytokine production by dispersed nasal polyp
cells (DNPCs).
Methods: DNPCs were pretreated with or without Poly(IC), and were then cultured in the presence or
absence of staphylococcal enterotoxin B (SEB), following which the levels of IL-5, IL-10, IL-13, IL-17A and
interferon (IFN)-g in the supernatant were measured. To determine the involvement of IL-10 and
cyclooxygenase in Poly(IC)-mediated signaling, DNPCs were treated with anti-IL-10 monoclonal antibody
and diclofenac, the cyclooxygenase inhibitor, respectively. Poly(IC)-induced prostaglandin E2 (PGE2)
production was also determined.
Results: Exposure to Poly(IC) induced a significant production of IL-10, but not of IL-5, IL-13, IL-17A or
IFN-g by DNPCs. Pretreatment with Poly(IC) dose-dependently inhibited SEB-induced IL-5, IL-13 and IL-
17A, but not IFN-g production. Neutralization of IL-10 significantly abrogated the inhibitory effect of
Poly(IC). Treatment with diclofenac also abrogated the inhibitory effect of Poly(IC) on SEB-induced IL-5
and IL-13 production. However, unlike exposure of diclofenac-treated DNPCs to lipopolysaccharide, the
ligand for TLR4, exposure of these cells to Poly(IC) did not enhance IL-5 or IL-13 production. Poly(IC) did
not significantly increase PGE2 production by DNPCs.
Conclusions: These results suggest that TLR3 signaling regulates eosinophilia-associated cytokine production
in CRSwNP, at least in part, via IL-10 production.