چکیده (انگلیسی):

The purpose of this study was to evaluate the effect of process (homogenization speed and
evaporation time) and formulation (aqueous/organic phase ratio, surfactant concentration,
polymer type and concentration, and drug amount) variables on the preparation of
paclitaxel-loaded biodegradable polymeric nanoparticles using modified solvent evaporation
technique.Thereafter, a formulation was selected and subjected to evaluation of inclusion
of a co-surfactant for further reduction of particle size. Particle size, encapsulation efficiency
and in-vitro drug release kinetics were evaluated. It was observed that the inclusion
of vitamin E TPGS (0.01%), Poloxamer 188 (0.5%) or Tween 80 (0.25%) reduced the particle
size of nanoparticles to 230, 244 or 301 nm from 438 nm, respectively. Encapsulation efficiency
increased for both vitamin E TPGS and Poloxamer 188 up to concentration at 0.010%
and 0.25%, respectively, while this was not the case for Tween 80. Comparison of drug release
kinetics demonstrated that drug release accelerated from paclitaxel-loaded biodegradable
nanoparticles prepared with the inclusion of Tween 80 but was delayed for Poloxamer 188
and vitamin E TPGS. Thus, it was concluded that the particle size of the nanoparticles could
be reduced further and the paclitaxel release kinetics could easily be adjusted by taking
advantage by the inclusion of a co-surfactant.