چکیده (انگلیسی):

Objective: To determine the toxic effect of diethanolamine on testicular steroidogenesis
and its possible amelioration by curcumin in mice.
Methods: Adult Swiss strain male albino mice were orally administered with diethanolamine
(110, 165 and 330 mg/kg body weight) for 45 days. In another set of
experiment, male albino mice were orally administered with curcumin (10, 25 and 50 mg/
kg body weight) along with diethanolamine (330 mg/kg body weight) for 45 days. On
completion of treatment, animals were humanely sacrificed, testis dissected out, blotted
free of blood and used for biochemical analysis. The obtained serum samples were
analyzed for serum testosterone level.
Results: Diethanolamine caused significant (P < 0.05), dose-dependent reduction in total
lipid and cholesterol contents, activities of 3b- and 17b-hydroxysteroid dehydrogenases
in testis as well as significant reduction in serum testosterone level. However, cotreatment
of curcumin along with diethanolamine caused significant (P < 0.05) dosedependent
amelioration in diethanolamine-induced changes in total lipid and cholesterol
contents, activities of 3b- and 17b-hydroxysteroid dehydrogenases and serum
testosterone levels.
Conclusions: The results of the present study showed that curcumin significantly ameliorates
diethanolamine reduced testicular steroidogenesis.