چکیده (انگلیسی):

Cisplatin (CP) is one of the most active cytotoxic drugs. However, it has several side effects
that are associated with increased oxidative stress. Aged garlic extract (AGE) is a natural
product containing different compounds with antioxidant activity. The present study
aimed to evaluate the effect of AGE on CP-induced hepatotoxicity. Four equally male rat
groups: control, AGE-treated (250 mg/kg once for 21 days), CP-treated (7.5 mg/kg, once
intraperitoneal), combined AGE and CP-treated were used. Blood samples were collected
to investigate blood picture and serum levels of aspartate aminotransferase (AST), alanine
aminotransferase (ALT), bilirubin (TSB) and albumin. The liver of each rat was excised,
cleaned, weighed, rinsed in ice-cold saline and homogenized for assessment malondialdehyde
(MDA) level, catalase (CAT) and superoxide dismutase (SOD) activities and level of
reduced glutathione (GSH). Histological examination was also carried out. AGE-pretreated
rats revealed significant reduction in serum levels of AST, ALT & TSB and increase serum
albumin level induced by CP administration. Furthermore, AGE significantly ameliorated
CP-induced increase in MDA level and decrease in GSH level, CAT and SOD activities in
liver tissue homogenates. Additionally, histopathological and blood picture examinations
revealed markedly ameliorated CP-induced toxicity on blood cells parameters and liver
structure. Our results prove that AGE has antioxidant and protective effects against CPinduced
oxidative stress and changes in parameters of blood cells and liver structure in
rats. Thus, it could be used as a dietary supplementation to reduce toxic side effects of
anticancer drugs.