چکیده (انگلیسی):

Estrogens, and particularly glucuronides such as ethinylestradiol (EE), have been shown to
cause cholestasis in animal studies, by reducing bile acid uptake by hepatocytes. The aim of the present
article is to investigate anticholestatic activity of the ethanolic extract of the aerial parts of Jasonia montana
against liver cholestasis induced by EE in adult female rats in an attempt to understand its mechanism
of action, which may pave the way for possible therapeutic applications. Subcutaneous
administration of 100 lg/kg b.w. ethinylestradiol to rats induced hepatocellular cholestasis with a significant
decrease in serum cholesterol, bile acids and bilirubin levels as well as in hepatic superoxide
dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) activities and hepatic
total, protein-bound and non-protein sulfhydryl groups. Also, treatment with EE produced significant
increase in serum Pi-glutathione-s-transferase (Pi-GST), gamma glutamyl transpeptidase (c-GT) and
alpha-glutathione-s-transferase (a-GST) activities as well as serum nitric oxide (NO) and tumor necrosis
factor alpha (TNF-a) level and hepatic malondialdehyde (MDA) level as compare to control group.
Oral administration of the aerial parts of ethanolic extract at a concentration of 150 mg/kg b.w. daily to
rats treated with EE for 15 days showed a significant protection against-induced decrease in serumcholesterol,
bile acids and bilirubin levels. The treatment also resulted in a significant increase in hepatic
SOD, GPx and GR activities as well as hepatic total, protein-bound and non-protein sulfhydryl
groups. In addition, the extract could inhibit serum Pi-GST, c-GT and a-GST activities as well as
reduce serum TNF-a,NO and hepaticMDAas compare to ethinylestradiol treated rats. High content
of flavonoids and phenolic compounds was found in ethanolic extract, which may be responsible for
free radical activity. The results clearly suggest that the aerial parts of J. montana extract may effectively
normalize the impaired antioxidant status in ethinylestradiol (EE)-cholestatic model. Thus the extract
may have a therapeutic value in drug-induced biliary cholestasis as well as in hormonal therapy.