چکیده (انگلیسی):

The effects of optimization condition on solid state fermentation of agro-allied waste for production of amylase using Bacillus sp was investigated. The amylase producing specie, was isolated from soil samples, rotten potatoe and spoiled fruit waste. Optimization of the amylase production using various agro-allied waste such as wheat bran, potatoe peel, Banana peel and rice bran were carried out. All the Bacillus sp isolates were screened by activity zone techniques with iodine solution. Out of 6 bacterial specie, only four (4) of the isolates showed positive results for amylase production. Bacterial strain-3 was found maximum at pH 6. The result showed that potatoe peel produced more enzyme activity under optimization conditions using Tap water, maltose, at pH 6, enzyme activity in the range of 0.555 iu/ml, 0.365 iu/ml, and 0.364 iu/ml respectively. Rice bran produced more enzyme activity of 0.342 iu/ml at 30°C while wheat bran showed an increased enzyme activity using peptone as source of nitrogen at 0.371 iu/ml, than any of the other agro-allied waste used for the amylase production. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on.