چکیده (انگلیسی):

Objective: To study the effect of adding different concentrations of the pomegranate
juice (PJ) to the cattle bull semen extender on post-thawing semen quality.
Methods: Semen was collected from five cattle-bulls at weekly intervals for 5 weeks at
the Semen Freezing Center, General Organization for Vet. Services, Ministry of Agriculture.
Semen samples were diluted in Tris-citric acid-egg yolk-fructose extender and
divided into six aliquots, the 1st served as control while PJ was supplemented at 10%,
20%, 30%, 40% and 50% in the aliquot 2, 3, 4, 5 and 6 respectively. Diluted semen
samples were subjected to cooling and cryopreservation and stored in liquid nitrogen
(LN2). Sperm motility in chilled semen (over 10 d) and post-thawing sperm parameters,
including individual motility, alive sperm, membrane integrity, and total sperm abnormality
were assessed.
Results: Obtained results clearly demonstrated that the addition of 10% PJ in the chilled
extended cattle semen proved to be beneficial for maintaining sperm motility percentage.
On the other hand, the addition of 40% and 50% PJ failed to preserve motility all over the
10 d. Also, supplementation of extender with 10–20% PJ significantly increases the postthaw
motility and viability as compared with control group.
Conclusions: Supplementation of bull semen extender with 10% and 20% PJ provides
good chilling and improved frozen-thawed semen quality.