چکیده (انگلیسی):

Cultured lung cells provide an alternative to animal exposures for comparing the effects of different
types of air pollution particles. Studies of particulate matter in vitro have reported proinflammatory
cytokine signaling in response to many types of environmental particles, but there have been few
studies comparing identical treatments in multiple cell types or identical cells with alternative cell
culture protocols. We compared soil-derived, diesel, coal fly ash, titanium dioxide, and kaolin
particles along with soluble vanadium and lipopolysaccharide, applied to airway-derived cells grown
in submerged culture. Cell types included A549, BEAS-2B, RAW 264.7, and primary macrophages.
The cell culture models (specific combinations of cell types and culture conditions) were
reproducibly different in the cytokine signaling responses to the suite of treatments. Further,
Interleukin-6 (IL-6) response to the treatments changed when the same cells, BEAS-2B, were grown
in KGM versus LHC-9 media or in media containing bovine serum. The effect of changing media
composition was reversible over multiple changes of media type. Other variables tested included
culture well size and degree of confluence. The observation that sensitivity of a cell type to
environmental agonists can be manipulated by modifying culture conditions suggests a novel
approach for studying biochemical mechanisms of particle toxicity.