چکیده (انگلیسی):

To evaluate the effects of the insulin-like growth factor I (IGF-I) complex from seminal
plasma on capacitation, membrane integrity and DNA fragmentation.
Methods: A total of 0.5 mL of fresh semen was added to 1 mL of Bracket–Oliphant
(BO) medium, and the sample was then centrifuged at a speed of 1800 rpm for 10 min.
The samples were analyzed before and after centrifugation for sperm viability, motility,
membrane integrity and capacitation. The centrifuged samples were divided into three
groups, each consisting of 3 × 106 spermatozoa. BO medium was added to group 1,
BO + 12 ng IGF-I complex medium was added to group 2, and 12 ng IGF-I complex was
added to group 3. Then, the samples were incubated for 15 min.
Results: The result showed that sperm motility, viability and membrane integrity were
significantly higher (P < 0.05) after centrifugation. Furthermore, the sperm capacitation
was significantly lower (P < 0.05) after centrifugation. The percentages of sperm
capacitation, membrane integrity and DNA fragmentation were significantly different
(P < 0.05) in all media, including BO, BO + IGF-I complex and the IGF-I complex alone.
Conclusions: Sperm quality include motility, viability and membrane integrity were
lower after centrifugation. Whereas DNA fragmentation after incubation in the IGF-I
complex medium also was lower compared to that of specimens in the BO and
BO + IGF-I complex media.