چکیده (انگلیسی):

Objective: Auto-regeneration of the intestinal and ovarian tissue are experiencing
degenerative due to protein energy malnutrition (PEM) through of auto-mobilization,
increase of immune response and differentiation of endogenous stem cells.
Methods: Female rat model of PEM obtained through fasting meals for 5 d and only to
drink, causing malnutrition and damage of the intestinal and ovarian tissue. Furthermore,
the therapy of honey with a dose of 30% water (T1) and 50% water (T2), respectively for
5 d and compared with the positive control, were fasted without being given honey (T0+)
and negative control, not fasted and without being given honey (T0−). Observations on:
auto-mobilization, increased immune response, differentiation of stem cells, and regeneration
of the intestinal and ovarian tissue with HE staining.
Results: Auto-mobilization of stem cells based on the expression of CD34+ and CD45+,
which is a marker of endogenous stem cells (hematopoietic stem cells/HSCs). Increased
immune response is based on Hsp70 expression and PGE2 in intestinal tissue. Differentiation
of stem cells into progenitor cells that expected based expression of growth
differentiation factor-9 (GDF-9) by immunohistochemistry in ovarian tissue.
Conclusions: Expression of CD34+ and CD45+, which significantly different in treatment
2 (2). Furthermore, increase of immune response (decrease Hsp70 expression and
increased PGE2) in intestinal tissue. Increased immune response causes expression of
GDF-9 in ovarian tissue. Decreased of Hsp70 expression, increased PGE2 and increased
GDF-9 followed the process of regeneration of the intestinal and ovarian tissue.