چکیده (انگلیسی):

Brassica napus (Bn) microspore-derived embryogenesis has become a model system to
study basic aspects of plant development. Recognized transcription factors governing embryogenesis
include: FUSCA3 (FUS3), a member of the plant-specific B3-domain family, and LEAFY
COTYLEDON1 (LEC1), a member of the HAP3 subunit of the CCAAT binding factor family.
The effects of altered expression of both genes were investigated during microspore-derived
embryogenesis in established B. napus lines over-expressing or down-regulating BnLEC1, as well
as in tilling lines where BnFUS3 was mutated. While over-expression of BnLEC1 decreases the yield
of microspore-derived embryos (MDEs) without affecting their ability to regenerate plants, suppression
of BnLEC1 or BnFUS3 reduced both embryo number and regeneration frequency.
Embryos produced by these lines showed structural abnormalities accompanied by alterations in
the expression of several embryogenesis-marker genes. Oil accumulation was also altered in the
transgenic MDEs. Total oil content was increased in MDEs over-expressing BnLEC1 and decreased
in those suppressing BnLEC1 or BnFUS3. Mutation of BnFUS3 also resulted in a small but significant
increase in linoleic (C18:2) acid. Together this study demonstrates the crucial role of BnLEC1
and BnFUS3 during in vitro embryogenesis.

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