چکیده (انگلیسی):

Objective: To compare two suites of culture media (one with HSA and one protein-free
(PF) supplemented with methylcellulose) for washing human sperm in IVF.
Methods: Semen samples (n = 41) underwent parallel density gradient preparation using
PF or HSA-supplemented culture medium and subsequent yield, survival, morphology
and motility were compared.
Results: The PF medium resulted in a significantly higher sperm yield (P < 0.0001), but
similar sperm morphology (P = 0.822) and 24-h survival (P = 0.11). There was, however,
a lower percentage of progressively motile sperm (P < 0.0001) and a higher proportion of
sperm demonstrating non-progressive motility (P < 0.0001) in the PF medium when
observed on a Makler Chamber, apparently an artefact as a similar sperm motility index
was measured using a Sperm Quality Analyser (P = 0.83). Attachment of sperm in PF
medium to the glass chamber reduced with time and any differences had disappeared after
6 min on the counting chamber.
Conclusion: These results support the use of PF media supplemented with methylcellulose
as an alternative to HSA, although a modification to the manufacturer's protocol of
6-min pre-incubation before assessing sperm motility must be used. Further studies
should investigate the function of such sperm prepared in PF medium.