چکیده (انگلیسی):

The aim of this research was to formulate Marsupsin–phospholipid complex (M–P Complex) in
attempt to increase the bioavailability of marsupsin and to characterize this new formulation along with
its evaluation. Marsupsin–phospholipid complex was formulated by mechanical dispersion method. In
this new formulation, complex formation was confirmed by carrying out transmission electron
microscopy (TEM), IR, 1H-NMR and RP-HPLC analysis. TEM showed M–P Complex diameter range
of 0.05–0.5 μm. The entrapment efficiency of M–P Complex was found to be 44%. In vitro release study
revealed its first order release profile. Mean blood serum concentration vs time curve of marsupsin was
of first order after oral administration of M–P Complex in albino rabbits which clearly showed
remarkably increased bioavailability of M–P Complex than standardized marsupsin. The average value
of Cmax and Tmax of M–P Complex were found to be 3.02 mg/ml and 10.2 h, respectively. Hence the
findings demonstrate that complexing marsupsin with phospholipids results in better oral bioavailability
and improved biological response than free form of standardized marsupsin.