چکیده (انگلیسی):

A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, andits -xylosidases optimization in response to agro-industrial residues was performed. Usingstatistical approach as a strategy for optimization, the induction of -xylosidases activitywas evaluated in residual corn straw, and improved so that the optimum condition achievedhigh -xylosidases activities 1003 U/mL. According our known, this study is the first to showso high levels of -xylosidases activities induction. In addition, the application of an experi-mental design with this microorganism to induce -xylosidases has not been reported untilthe present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60◦Cshowing better thermostability at 55◦C. The saccharification ability of -xylosidase in thepresence of hemicellulose obtained from corn straw raw and xylan from beechwood sub-strates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively,at 50◦C. Our data strongly indicated that the -xylosidases activities was not subjected to theeffects of potential enzyme inhibitors often produced during fermentation process. Thesedata suggest the application of this enzyme studied for saccharification of hemicellulose,an abundant residue in the American continents, thus providing an interesting alternativefor future tests for energy production.© 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This isan open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).