چکیده (انگلیسی):

The aim of this study is to evaluate the effect of moderate physical exercise and treatment
time on the organism’s response to NSC631570.The sensitivity of circulating phagocytes
to the drug at different times of day was estimated in in vitro experiments. NSC631570 was
administered intravenously to healthy volunteers (eleven men, 23 ± 2 years) in a single therapeutic
dose in an inpatient and an outpatient setting. Blood samples were obtained before
the drug administration, 30 min after the drug injection and every fourth hour throughout
the 24 hour period. Biochemical parameters were determined using the hematological analyzer.
Flow cytometry was used to evaluate phagocyte metabolism.Treatment of circulating
phagocytes with NSC631570 in vitro resulted in an increase in ROS production along with a
decrease in their phagocytic activity, most expressed in the morning time. Drug injection
to sedentary persons resulted in pro-inflammatory metabolic polarization of circulating phagocytes.
Introduction of NSC631570 to active persons was accompanied by a significant increase
in phagocyte endocytosis along with a decrease in the daily mean of ROS generation. Significant
oscillation (but in the normal ranges) of urea, creatinine, alanine aminotransferase
and aspartate aminotransferase after NSC631570 introduction in the outpatient setting was
shown during the day. Physical activity interferes with immunomodulatory action of
NSC631570 and abrogates pro-inflammatory shift of circulating phagocytes. Biochemical parameters
of blood from patients treated with NSC631570 in the outpatient setting must be
interpreted cautiously considering the effect of physical activity on some metabolic biomarkers.