چکیده (انگلیسی):

A sensitive and simple fluorimetric method was developed for the determination of
diosmin and hesperidin. The proposed method involves the formation of ternary complex with
Tb3+ in the presence of Tris buffer. The fluorescence quenching of Tb3+ at 549 and 494 nm (kex
at 275 and 248 nm) due to the complex formation was quantitatively measured for diosmin and
hesperidin, respectively. The reaction conditions and the fluorescence spectral properties of the
complexes have been investigated. Under the described conditions, the proposed method was applicable
over the concentration range (4.93 · 106–1.81 · 105 mol) and (3.28 · 106–1.64 · 105 mol)
with mean percentage recoveries 100.22± 0.89 and 99.13± 0.72 for diosmin and hesperidin,
respectively. The proposed method was applied successfully for the determination of studied drugs
in bulk powder, dosage forms and plasma samples. The results obtained by applying the described
method were statistically analyzed and compared with those obtained