چکیده (انگلیسی):

Objective: To discuss the effect and mechanism of miR-34a on the proliferation,
apoptosis and invasion of laryngeal carcinoma cells.
Methods: The laryngeal squamous carcinoma Hep2 cells were transiently transfected
with miR-34a mimics and miR-34a NC. The MTT, colony-forming assay, Hoechst
staining and AnnexinV-PI double staining flow cytometry were employed to detect the
effect of miR-34a on the viability and apoptosis of laryngeal squamous carcinoma Hep2
cells; Transwell assay to defect the effect of miR-34a on the migration and invasion of
laryngeal squamous carcinoma Hep2 cells; western blot and RT-PCR assay to defect the
effect of miR-34a mimics on the expression of survivin and Ki-67 mRNA in laryngeal
squamous carcinoma Hep2 cells.
Results: Compared with miR-34a NC group, the cell viability in miR-34mimics group was
significantly decreased (P < 0.01), the cell apoptosis rate was significantly increased
(P < 0.01), the abilities of cell migration and invasion were significantly reduced (P < 0.01)
and the expression of survivin and Ki-67 mRNA was significantly decreased (P < 0.01).
Conclusions: The increased expression of miR-34a can induce the apoptosis of Hep2
laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related
to the down-regulated expression of survivin and Ki-67.