چکیده (انگلیسی):

Objective: To investigate the role of survivin in osteosarcoma metastasis.
Methods: Small interfering RNA (siRNA) was used to knockdown the expression of
survivin and a5 integrin in the human osteosarcoma cell line MG63. Western blotting and
immunostaining methods were used to assessed the effect of survivin knockdown on the
expression of a5 integrin through flow cytometry and fluorescence microscopy detection.
Meanwhile, the invasion and migration of transfected cells in Transwell and wound
healing assays were probed, and the growth situation of these cells transplanted into nude
mice was monitored.
Results: Knockdown of survivin expression could inhibit the invasion and migration of
osteosarcoma MG64 cells in vitro and the expression of a5 integrin on osteosarcoma
MG64 cell surface, suggesting that survivin can inhibit the invasion and migration of
osteosarcoma cells through downregulation of a5 integrin. Anti-a5 integrin antibody
could also markedly decrease the capability of invasion and migration of osteosarcoma
MG64 cells. Additionally, knockdown of survivin expression could slow the growth of
osteosarcoma MG63 cells transplanted into nude mice.
Conclusions: Survivin-directed anti-tumor strategies might be an effective method in the
treatment of osteosarcoma.