چکیده (انگلیسی):

Objective: To investigate the effect of Gymnema sylvestre extract (GS) on initial antiobesity,
liver injury, and glucose homeostasis induced by a high-fat diet (HFD).
Methods: The dry powder of GS was extracted with methanol, and gymnemic acid was
identified by high performance liquid chromatography as deacyl gymnemic acid. Male
C57BL/6J mice that fed on either a normal diet, normal diet containing 1 g/kg GS
(CON+GS), HFD, or HFDcontaining 1.0 g/kg GS (HFD+ GS) for 4 weeks were used to test
the initial anti-obesity effect ofGS. Body weight gain and food intake, and serum levels about
lipid and liver injury markers were measured. Histopathology of adipose tissue and liver
stained with hematoxylin and eosin (H&E) and oil-redOwere analyzed. After 4 weeks of GS
extract feeding, intraperitoneal glucose tolerance test (IPGTT) was performed.
Results: The methanol extracts of GS exerted significant anti-obesity effects in HFD + GS
group. They decreased body weight gain, a lower food and energy efficiency ratio, and
showed lower serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein
(LDL)-cholesterol, very-low density lipoprotein (VLDL)-cholesterol and leptin
compared with theHFDgroup. The decreases of abdominal as well as epididymal fat weight
and adipocyte hypertrophy, lipid droplets in liver, and serum levels of aspartate aminotransferase
(AST) and alanine transaminase (ALT) were also observed. The CON + GS
group showed an effect of glucose homeostasis compared to the CON group.
Conclusions: This study shows that GS provide the possibility as a key role in an initial
anti-obesity effects feeding with a HFD.