چکیده (انگلیسی):

Background/objective: Hematopoietic stem cell transplant (HSCT) is the only curative treatment
for myeloproliferative neoplasm-associated myelofibrosis (MPN-MF). The main clinical
manifestation of MPN-MF is splenomegaly secondary to extramedullary hematopoiesis (EMH).
The effects of HSCT on splenic EMH and associated vascular and stromal changes are unknown.
This study compares the findings seen in spleens following HSCT with those of nontransplanted
patients, normal controls, and matched bone marrow (BM) samples.
Methods: This study included three transplanted MPN-MF spleens, three nontransplanted MPNMF
spleens, and three normal controls. Spleens were assessed for: (a) presence/extent of EMH;
(b) presence of Gamna–Gandy bodies; (c) splenic fibrosis; (d) CD34-positive microvessel density;
(e) CD8-positive sinusoids; (f) frequency of smooth muscle actin-positive myoid cells;
and (g) nerve growth factor receptor-positive adventitial reticulum cells. In two cases,
http://dx.doi.org/10.1016/j.hemonc.2016.07.002
1658-3876/
2016 King Faisal Specialist Hospital & Research Centre. Published by Elsevier Ltd.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Abbreviations: BM, bone marrow; CR, complete remission; ET, essential thrombocythemia; HSCT, hematopoietic stem cell transplant; MF,
myelofibrosis; MPN, myeloproliferative neoplasm; PMF, primary myelofibrosis; PV, polycythemia vera; RIC, reduced intensity conditioning
* Corresponding author at: Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, Room ST-707,
525 East 68th Street, New York, NY 10065, USA. Fax: +1 212 746 2009.
E-mail address: ato9002@med.cornell.edu (A. Orazi).