سلول جدید ESS@ ,مکمل استفاده شده بعنوان تغذیه یا اولین تقویت کننده به CHOکه منتج شده است به ارتقامناسب دربازده پروتئین و تولیدات است
Novel Cell-Ess ® supplement used as a feed or as an initial boost to CHO serum free media results in a significant increase in protein yield and production
نویسندگان |
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اطلاعات مجله |
Computational and Structural Biotechnology Journal .volume14.journal homepage: www.elsevier.com/locate/csbj |
سال انتشار |
2016 |
فرمت فایل |
PDF |
کد مقاله |
5026 |
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چکیده (انگلیسی):
Many metrics, including metabolic profiles, have been used to analyze cell health and optimize productivity. In
this study, we investigated the ability of a lipid supplement to increase protein yield. At a concentration of 1%
(v/v) the lipid supplement caused a significant increase in protein titer (1118 ± 65.4 ng 105 cells−1 days−1)
when compared to cultures grown in the absence of supplementation (819.3 ± 38.1 ng 105 cells−1 days−1;
p b 0.05). This equated to a 37% increase in productivity. Furthermore, metabolic profiles of ammonia, glutamate,
lactate, and glucosewere not significantly altered by the polar lipid supplement. In a separate set of experiments,
using the supplement as a feed resulted in 2 notable effects. The first was a 25% increase in protein titer. The
second was an extension of peak protein production from 1 day to 2 days. These results suggest that lipid
supplementation is a promising avenue for enhancing protein production. In addition, our results also suggest
that an increase in protein production may not necessarily require a change in the metabolic state of the cells.
© 2016 The Author. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural
Biotechnology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.
کلمات کلیدی مقاله (فارسی):
پروسه زیستی.چگالی محلول.لیپید.سلولESS.انتی بادی مونوکلونالCHO.واسطه های ازادسرم.ترکیبات ازادحیوانات.تراکم سلول زنده.واکنش گرزیستی.
کلمات کلیدی مقاله (انگلیسی):
Bioprocessing. Titer. Lipid. Cell-Ess. CHO Monoclonal antibody. Serum free media. Animal component free. Viable cell density. Bioreactor .Single use bioreactor
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