چکیده (انگلیسی):

Many metrics, including metabolic profiles, have been used to analyze cell health and optimize productivity. In
this study, we investigated the ability of a lipid supplement to increase protein yield. At a concentration of 1%
(v/v) the lipid supplement caused a significant increase in protein titer (1118 ± 65.4 ng 105 cells−1 days−1)
when compared to cultures grown in the absence of supplementation (819.3 ± 38.1 ng 105 cells−1 days−1;
p b 0.05). This equated to a 37% increase in productivity. Furthermore, metabolic profiles of ammonia, glutamate,
lactate, and glucosewere not significantly altered by the polar lipid supplement. In a separate set of experiments,
using the supplement as a feed resulted in 2 notable effects. The first was a 25% increase in protein titer. The
second was an extension of peak protein production from 1 day to 2 days. These results suggest that lipid
supplementation is a promising avenue for enhancing protein production. In addition, our results also suggest
that an increase in protein production may not necessarily require a change in the metabolic state of the cells.
© 2016 The Author. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural
Biotechnology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.