چکیده (انگلیسی):

The increased number of annotated bacterial genomes provides a vast resource for genome mining. Several
bacterial natural products with epoxide groups have been identified as pre-mRNA spliceosome inhibitors
and antitumor compounds through genome mining. These epoxide-containing natural products feature
a common biosynthetic characteristic that cytochrome P450s (CYPs) and its patterns such as epoxidases
are employed in the tailoring reactions. The tailoring enzyme patterns are essential to both biological
activities and structural diversity of natural products, and can be used for enzyme pattern-based genome
mining. Recent development of direct cloning, heterologous expression, manipulation of the biosynthetic
pathways and the CRISPR-CAS9 system have provided molecular biology tools to turn on or pull out
nascent biosynthetic gene clusters to generate a microbial natural product library. This review focuses
on a library of epoxide-containing natural products and their associated CYPs, with the intention to provide
strategies on diversifying the structures of CYP-catalyzed bioactive natural products. It is conceivable that
a library of diversified bioactive natural products will be created by pattern-based genome mining, direct
cloning and heterologous expression as well as the genomic manipulation.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-
nd/4.0/).