چکیده (انگلیسی):

Objective: To discover the effect of partial splenic embolization on the immune function
of cirrhotic patients with hypersplenism.
Methods: Patients involved in the study were enrolled and divided into three groups,
including control group, experimental group, and complication group. Numbers of CD3+,
CD4+ and CD8+ T cells and CD4+CD25+CDl27low/− Treg cells in the peripheral blood of
patients before surgery, 1 month, 6 months, 1 year, and 2 years after surgery were
analyzed by fluorescence active cell sorting (FACS). Contents of immunoglobulins (IgA,
IgG and IgM) were analyzed by auto immunoassay analyzer.
Results: In the peripheral blood of patients from experimental group, numbers of CD3+,
CD4+ and CD8+ T cells initially declined, but afterwards increased to normal level; in the
peripheral blood of patients from complication group, CD3+ and CD8+ T cells showed the
same trend, but the number of CD4+ T cells was below normal level at all detection times.
Furthermore, CD3+, CD4+ and CD8+ T cells in the peripheral blood of patients from
complication group were initially less than those in experimental group, and afterwards
were comparable between two groups. In patients from both experimental group and
complication group, the number of CD4+ CD25+ CDl27low/−Treg cells increased 1 month
and 6 months after surgery, and gradually restored to normal level.
CD4+CD25+CDl27low/− Treg cell counts in patients from complication group were
initially more than those in patients from experimental group 1 month and 6 months after
surgery, but then they were comparable. Furthermore, contents of immunoglobulins (IgA,
IgG and IgM) were comparable in three groups at all detection times.
Conclusion: Partial splenic embolization influenced the immune function of cirrhotic
patients with hypersplenism in the short term but the immune function could afterwards
gradually restore to normal. Our results implicated that measures that prevent infection
and improve immune function were necessary in early stage after undergoing PSE in
order to reduce complications.