چکیده (انگلیسی):

The aim of this study was to evaluate and compare the in
vitro and in vivo transdermal potential of w/o microemulsion
(M) and gel (G) bases for diclofenac sodium (DS). The effect
of dimethyl sulfoxide (DMSO) as a penetration enhancer
was also examined when it was added to the M formulation.
To study the in vitro potential of these formulations, permeation
studies were performed with Franz diffusion cells
using excised dorsal rat skin. To investigate their in vivo
performance, a carrageenan-induced rat paw edema model
was used. The commercial formulation of DS (C) was used
as a reference formulation. The results of the in vitro permeation
studies and the paw edema tests were analyzed by
repeated-measures analysis of variance. The in vitro permeation
studies found that M was superior to G and C and that
adding DMSO to M increased the permeation rate. The
permeability coefficients (Kp) of DS from M and M+DMSO
were higher (Kp = 4.9 × 10−3 ± 3.6 × 10−4 cm/h and 5.3 ×
10−3 ± 1.2 × 10−3 cm/h, respectively) than the Kp of DS
from C (Kp = 2.7 × 10−3 ± 7.3 × 10−4 cm/h) and G (Kp =
4.5 × 10−3 ± 4.5 × 10−5 cm/h). In the paw edema test, M
showed the best permeation and effectiveness, andM+DMSO
had nearly the same effect as M. The in vitro and in vivo
studies showed that M could be a new, alternative dosage
form for effective therapy.