چکیده (انگلیسی):

Objective: To investigate droplet medium volume effect on the BMP-15 m-RNA
expression.
Methods: Oocytes are collected from mice ovaries by puncturing with a sterile 26-G
needle. The droplet medium volumes are using 50 mL, 100 mL and 200 mL. The BMP-
15 m-RNA expression is determined in each group.
Results: The results indicated that BMP-15 m-RNA expression did not significantly
difference when oocyte were cultured in 50 and 100 mL/droplet medium volume, but
significantly difference (P < 0.05) when oocytes were cultured in 200 mL/droplet medium
volume.
Conclusions: The highest BMP-15 m-RNA expression occurs when oocytes are cultured
in 200 mL/droplet medium volume.