چکیده (انگلیسی):

Objective: An efficient in vitro plant regeneration protocol for Canscora decussata
Schult. (Gentianaceae) (C. decussate), a threatened medicinal herb used in Ayurvedic
system of medicine was developed. Seed germination was achieved on MS growth
regulator free medium.
Methods: The nodal explants were excised from the in vitro raised seedlings and
inoculated on MS medium supplemented with various plant growth regulators such as
BAP, KIN, TDZ and Zeatin individually and in combinations with or without GA3. BAP
(2 mg/L) was proved to be effective for multiple shoot induction (30.20 ± 6.53) among
the cytokinin tested individually. Addition of NAA (1 mg/L) to cytokinin containing
medium resulted in callus, KIN (3 mg/L) with NAA (1 mg/L) produced highest percentage
of callus (82%) per explant.
Results: Among the various combination of cytokinin tested, BAP (0.5 mg/L) in combination
with KIN (2 mg/L) induced highest number of multiple shoots (72.10 ± 1.05
shoot per explant). Addition of 1 mg/L of GA3 to the above medium induced highest
number of shoots (100.80 ± 3.20) with an average shoot length of 6.98 ± 0.66 cm.
Rooting was optimized in half-strength MS medium supplemented with IBA at 1.0 mg/L.
The plantlets were successfully transferred to hardening medium containing vermiculite
with 83% survival rate. Among the antioxidant activity of methanol extract of wild-grown
plants and in vitro regenerants tested, half-MS medium supplemented with NAA (0.5 mg/
L) derived callus has promising activity for total phenolics, DPPH, ABTS, FRAP and
phosphomolymbdenum assays. Total flavonoid content was found to be high in callus
derived from MS medium supplemented with KIN (2 mg/L) in combination with NAA
(1 mg/L).
Conclusion: Our present study suggest that in vitro derived callus of C. decussata
represent a promising alternative source to meet the pharmaceutical demands for commercial
formulations and the protocol could effectively be applied for the conservation of
C. decussata Schult.