چکیده (انگلیسی):

Objective: To display the effect of different concentrations of glycerol, as a cryopreservative,
on the quality of the frozen-thawed buffalo semen extended in TRIS
extender enriched with disaccharides (trehalose or sucrose).
Methods: Semen samples were extended in Tris-Citric acid-Fructose-Egg yolk without
addition of trehalose/sucrose and with 6.4% glycerol as a control (TFEG-C) and with the
addition of Trehalose/Sucrose and different concentrations of glycerol to ensure 60
million motile spermatozoa mL−1. Semen cooled slowly up to 5 and equilibrated for 4 h.
Semen was packed into 0.25 mL polyvinyl French straws. The straws were placed
horizontally on a rack and frozen in a vapor 4 cm above liquid nitrogen (LN2) for 10 min
then dipped in liquid LN2. Frozen straws were thawed at 37
C for 1 min. The parameters
studied were sperm motility, sperm viability, sperm abnormality, sperm membrane
integrity (HOST), percent of normal intact acrosome and DNA fragmentation.
Results: The best sperm motility, sperm liveability, sperm abnormality, sperm cell
membrane and DNA integrities appeared with TFES-G 5.5% (41.00 ± 2.08%,
70.40 ± 2.27%, 7.80 ± 1.19%, 68.10 ± 1.55%, 98.90 ± 0.50%, respectively) and TFES-G
7.3% (41.50 ± 1.98%, 70.70 ± 2.03%, 10.80 ± 0.88%, 69.30 ± 1.85% and 96.40 ± 0.88%,
respectively).
Conclusion: From the present study, it can be concluded that addition of glycerol (5.5%
or 7.3%) to Tris-Fructose-Egg yolk-Sucrose extender might help in improvement of the
post-thawed characteristics of buffalo frozen semen.